These data suggest that IL-6 is one important cytokine in the inductive differentiation of pluripotent ESCs toward hematopoiesis in vitro. Unlike the use of individual endothelial cell (EC) lines to study vascular formation, the EB vessel development model provides a three-dimensional microenvironment that mimics an in vivo milieu, including extracellular matrix, secreted factors, and surrounding non-EC cells, all of which. Although pooled samples of HUCS also contained granulocyte colony-stimulating factor (G-CSF), depletion of G-CSF from HUCS had no effect on either the percentage of embryoid bodies containing hematopoietic foci or the number of hematopoietic foci per embryoid body. Embryonic stem cell (ESC)-derived embryoid body (EB) is a unique model for studying vascular development. They are collectable from commercially obtainable mesenchymal cells such as human fibroblasts, bone. They reside in the connective tissue of nearly every organ including the umbilical cord, bone marrow and peripheral blood. Depletion of IL-6 from HUCS decreased the percentage of differentiated embryoid bodies containing hematopoietic islands, and decreased the number of hematopoietic foci per embryoid body as well. A Muse cell ( Mu lti-lineage differentiating s tress e nduring cell) is an endogenous non-cancerous pluripotent stem cell. Pooled HUCS samples were found to contain 10 to 13 pg/mL IL-6. When cultured in human umbilical cord serum (HUCS), pluripotent ESCs differentiate to embryoid bodies expressing tissue development equivalent to day 7-10 mouse embryos. EMBRYONIC STEM-CELL LINES - FORMATION OF VISCERAL YOLK-SAC, BLOOD ISLANDS AND.
![embryoid body blood island embryoid body blood island](http://www.webpathology.com/slides-13/slides/Testes_MixedGCT_Polyembryoma2.jpg)
The murine embryonic stem cell (ESC) in vitro differentiation system was used to study the role of interleukin-6 (IL-6) in the induction of early embryonic hematopoietic development. Embryoid body formation or addition of exogenous growth factors was not.